Genome‐wide identification of transcription factor binding sites with the ChIP‐seq method is an extremely important scientific endeavor − one that should ideally be performed for every transcription factor in as many cell types as possible. A major hurdle on the way to this goal is the necessity for a specific, ChIP‐grade antibody for each transcription factor of interest, which is often not available. Here, we describe CETCh‐seq, a recently published method utilizing genome engineering with the CRISPR/Cas9 system to circumvent the (...) need for a specific antibody. Using the CETCh‐seq method, targeted genomic editing results in an epitope‐tagged transcription factor, which is recognized by a well‐characterized, standard antibody, efficacious for ChIP‐seq. We have used CETCh‐seq in human cancer cell lines as well as mouse embryonic stem cells. We find that roughly 60% of transcription factors tagged using CETCh‐seq produce a high quality ChIP‐seq map, a significant improvement over traditional antibody‐based methods. (shrink)

In Rediscovering Colors: A Study in Pollyanna Realism, Michael Watkins endorses the Moorean view that colors are simple, non-reducible, properties of objects. Consequently, Watkins breaks from what has become the received view that either colors are reducible to certain properties of interest to science, or else nothing is really colored. What is novel about the work is that Watkins, unlike other Mooreans, takes seriously the metaphysics of colors. Consequently, Watkins provides an account of what colors are, (...) how they are related to the physical properties on which they supervene, and how colors can be causally efficacious without the threat of causal overdetermination. Along the way, he provides novel accounts of normal conditions and non-human color properties. The book will be of interest to any metaphysician and philosopher of mind interested in colors and color perception. (shrink)

It is widely acknowledged that interdisciplinarity (ID) is very diverse. Our contribution is a demonstration that considerable diversity exists also on the level of understandings of ID that researchers working in the same ID field express. Specifically, we analyse qualitatively, building on the method of culture contrast, six interviews with researchers working in computational linguistics and language technology in Estonia. We identify six understandings of ID expressed by the interviewees: centred on an ID method; a disciplinary method in an ID (...) field; an ID way of seeing and thinking; ID education; ID interests; one’s field as naturally ID. We show how understandings of ID are significant for analysing research practice, since they are involved in how researchers form a positive picture of themselves and their colleagues. We also show how an awareness of different understandings of ID is useful for discussing the significance of integration in ID. (shrink)

Before the story can be told, however, some stage-setting is necessary. First, it is important to be clear about the most basic doctrines of Pre-established Harmony, Occasionalism, and Physical Influx. Physical Influx asserts intersubstantial causation amongst finite substances. For instance, when I appear to kick a ball, I really am the cause of the ball's motion. Pre-established Harmony denies intersubstantial causation, but affirms intrasubstantial causation. According to Pre-established Harmony, then, I am not the cause of the ball's motion, but rather (...) the ball is simply causing itself to move. Occasionalism, like Pre-established Harmony, denies intersubstantial causation, but, unlike Pre-established Harmony, it denies intrasubstantial causation as well. Occasionalism typically asserts that God alone, that is, an infinite substance, is the cause of all changes, and thus of the ball's motion. Though there are other ways of defining these causal theories, this way has the advantage that the causal theories form an exhaustive disjunction. (shrink)

The A to Z of Kierkegaard's Philosophy provides a contextual introduction to Kierkegaard's 19th century world of Copenhagen, a chronology of events and key figures in his life, as well as definitions of the key systems of his thought-theology, existentialism, literature, and psychology. The extensive bibliographical section covers secondary literature and electronic materials of help to researchers. The appendix includes detailed information on his writings, along with a list of his pseudonyms.

As guidelines for neonatal resuscitation evolve from a growing evidence base, clinicians must ensure that practice is closely aligned with the available evidence, based on methodologically sound and ethically conducted research. This paper reviews ethical, legal and risk-management issues arising during the design of a quality-assurance project to make video recordings of neonatal resuscitations after high-risk deliveries. The issues, which affect patients, researchers, staff and the hospital at large, include the following: 1) Informed consent for research involving emergency procedures is (...) often not possible, for lack of time to provide sufficient information. The mental capacity of the subject or parent may be compromised by the impending emergency, and freedom of choice is threatened by the time pressure to consent. 2) Video recording of the inevitable medical errors raises issues of whether participating staff may be identifiable and accountable, affecting their willingness to participate in such research. The approach to staff participation and identification is reviewed. 3) The use of video data for education threatens the privacy of research subjects. The ethics of maintaining privacy is balanced with the ethics of using the data to improve practice of resuscitation. 4) The research subjects (patients, or the staff whose performance is being monitored) must be defined. 5) There are legal and ethical aspects of management and ownership of data. 6) The role of the Human Research Ethics Committee in protecting the research subject and possibly the medicolegal interests of the hospital is discussed. This paper reviews the literature and discusses the issues. (shrink)

Under the influence of Emmanuel Levinas and Jacques Derrida, the theme of absolute alterity still dominates the thinking of the ethical in Continental philosophy. This article examines an alternative ethical démarche, Jean-Luc Nancy's ‘singular plurality’, which refuses to start with the opposition of same and other, arguing instead for a primacy of relation, the ‘in-common’ and the ‘with’. The article first distinguishes Nancy's ‘singular plural’ from other recent attempts to disengage ethical thinking from the Levinasian framework, before showing how Nancy (...) proceeds otherwise than in terms of sameness and alterity while still maintaining an ethical impetus. Foregrounding what is politically and philosophically at stake in the difference between Nancy and the Levinasian/Derridean model, the article concludes by considering how Nancy can be defended against critics who mistakenly argue that he discounts alterity. (shrink)

According to the “adequacy-for-purpose” view of data evaluation, data should be evaluated as better or worse relative to a given research purpose and corresponding research context. In this paper, I apply the adequacy-for-purpose view to a novel case study—concerning the use of paleoecological data to make predictions about coral reef response to contemporary climate change—and then use the case study to suggest two extensions to the adequacy-for-purpose view. First, I argue that we can evaluate research purposes according to their productivity (...) (how well these research purposes serve other, more ultimate purposes). Second, I argue that we can also evaluate research purposes according to their plausibility (including whether we have access to data that are adequate for these research purposes). The relationship between data evaluation and purpose setting should be seen as an iterative one, in which both data and purposes are refined in concert over time. (shrink)

Name der Zeitschrift: Kant-Studien Jahrgang: 105 Heft: 4 Seiten: 471-490.

In his book The Retreat to Commitment Professor Bartley raised an important problem: can rationalism can rationalism be held in a rational way, that is, in a way that complies with its own requirements? Or is there bound to be something irrational in the rationalist's position? Briefly, Hartley's answer was that an element of irrationalism is involved in extant versions of rationalism; however, Bartley proposed a new version of rationalism that can, he claimed, be held in a way that is (...) rational according to its own account of rationality. Bartley called this ‘Comprehensively Critical Rationalism’. (shrink)

The first truly comprehensive and fully up-to-date critical introduction to the philosophy of Giorgio Agamben for an interdisciplinary audience.

By treating colours as sui generis intrinsic properties of objects we can maintain that (1) colours are causally responsible for colour experiences (and so agree with the physicalist) and (2) colours, along with the similarity and difference relations that colours bear to one another, are presented to us by casual observation (and so agree with the dispositionalist). The major obstacle for such a view is the causal overdetermination of colour experience. Borrowing and expanding on the works of Sydney Shoemaker and (...) Stephen Yablo, the paper offers a solution. (shrink)

This volume provides English translations of texts that form the essential background to Kant's Critique of Pure Reason. Presenting the projects of Kant's predecessors and contemporaries in eighteenth-century Germany, it enables readers to understand the positions that Kant might have identified with 'pure reason', the criticisms of pure reason that had developed prior to Kant's, and alternative attempts at synthesizing empiricist elements within a rationalist framework. The volume contains chapters on Christian Wolff, Martin Knutzen, Alexander Baumgarten, Christian Crusius, Leonhard Euler, (...) Johann Lambert, Marcus Herz, Johann Eberhard, and Johann Tetens. Each chapter includes a brief introduction that provides succinct biographical and bibliographical information on these authors, a concise account of their projects, and information on the importance of these projects to Kant's First Critique. Extensive references to the First Critique, brought together in a concordance, highlight the potential relevance of each text. (shrink)

The first critical work to attempt the mammoth undertaking of reading Badiou's Being and Event as part of a sequence has often surprising, occasionally controversial results. Looking back on its publication Badiou declared: “I had inscribed my name in the history of philosophy”. Later he was brave enough to admit that this inscription needed correction. The central elements of Badiou's philosophy only make sense when Being and Event is read through the corrective prism of its sequel, Logics of Worlds, published (...) nearly twenty years later. At the same time as presenting the only complete overview of Badiou's philosophical project, this book is also the first to draw out the central component of Badiou's ontology: indifference. Concentrating on its use across the core elements Being and Event-the void, the multiple, the set and the event-Watkin demonstrates that no account of Badiou's ontology is complete unless it accepts that Badiou's philosophy is primarily a presentation of indifferent being. Badiou and Indifferent Being provides a detailed and lively section by section reading of Badiou's foundational work. It is a seminal source text for all Badiou readers. (shrink)

During the last twenty years, Kant's theory of biology has increasingly attracted the attention of scholars and developed into a field which is growing rapidly in importance within Kant studies. The volume presents fifteen interpretative essays written by experts working in the field, covering topics from seventeenth- and eighteenth-century biological theories, the development of the philosophy of biology in Kant's writings, the theory of organisms in Kant's Critique of the Power of Judgment, and current perspectives on the teleology of nature.

For those open to the possibility that philosophical thought can improve life, David Hume's Essays: Moral, Political, and Literary have something to say. In the first comprehensive study of the Essays, Margaret Watkins engages closely with these neglected texts and shows how they provide important insights into Hume's perspective on the breadth and depth of human life, arguing that the Essays reveal his continued commitment to philosophy as a discipline that can promote both social and individual progress. Addressing topics (...) such as politics, war, slavery, the priesthood, the development of industry, aesthetics, emotional disorders, egoism, friendship, sexuality, gender relations, and the nature of philosophy itself, the volume examines Hume's purposes and aims against the backdrop of the eighteenth century society in which he lived. It will be of interest to scholars of modern thought in philosophy, politics, history, and economics. (shrink)

Even though Kant’s theory of cognition (Erkenntnis) is central to his Critique of Pure Reason, it has rarely been asked what exactly Kant means by the term “cognition”. Against the widespread assumption that cognition (in the most relevant sense of that term) can be identified with knowledge or if not, that knowledge is at least a species of cognition, we argue that the concepts of cognition and knowledge in Kant are not only distinct, but even disjunct. To show this, we (...) first (I) investigate Kant’s explicit characterizations of the nature of cognition. As it turns out, he introduces several different notions that must be carefully distinguished before identifying the one that is central to his project in the first Critique. We then (II) consider the basic features of Kant’s conception of knowledge, indicating both how it involves assent and objective justification and how it relates to our contemporary conception. Next (III), we compare and contrast Kant’s understanding of cognition and his conception of knowledge in a way that allows us to present their fundamental differences and connections. We argue that while cognition, in the most relevant sense, is a species of representation that differs from other representations in that it involves the conceptual determination of a sensibly given object, knowledge (for Kant) is a kind of assent to a judgment that requires consciousness of a sufficient epistemic ground. Finally (IV), by appreciating the differences between cognition and knowledge, we explain several of the implications this conception of cognition has for some of Kant’s main claims in the Critique of Pure Reason as a whole. Among other things, we show how Kant can deny cognition of specific things in themselves while allowing philosophical knowledge about things in themselves in general (e.g. that they exist, are not in space and time, etc.). (shrink)

Does it make sense to refer to bird song - a complex vocalization, full of repetitive and transformative patterns that are carefully calculated to woo a mate - as art? What about a pack of wolves howling in unison or the cacophony made by an entire rain forest? Redefining music as "the art of possibly animate things," Musical Vitalities charts a new path for music studies that blends musicological methods with perspectives drawn from the life sciences. In opposition to humanist (...) approaches that insist on a separation between culture and nature--approaches that appear increasingly untenable in an era defined by human-generated climate change--Musical Vitalities treats music as one example of the cultural practices and biotic arts of the animal kingdom rather than as a phenomenon categorically distinct from nonhuman forms of sonic expression. The book challenges the human exceptionalism that has allowed musicologists to overlook music's structural resemblances to the songs of nonhuman species, the intricacies of music's physiological impact on listeners, and the many analogues between music's formal processes and those of the dynamic natural world. Through close readings of Austro-German music and aesthetic writings that suggest wide-ranging analogies between music and nature, Musical Vitalities seeks to both rekindle the critical potential of nineteenth-century music and rejoin the humans at the center of the humanities with the nonhumans whose evolutionary endowments and planetary fates they share. (shrink)

Recent criticisms of non-reductive accounts of color assume that the only arguments for such accounts are a priori arguments. I put forward a posteriori arguments for a non-reductive account of colors which avoids those criticisms.

Object of this reply. A chap like myself, who struggles along with an amateur's box of logical tools, is bound to feel uneasy when his arguments are probed by the kind of logical precision-instruments which Professor Hempel manipulates so effortlessly. Yet after painstakingly working over his technical arguments, and after appealing for expert assistance on matters outside my competence,1 I have reached the surprising and agreeable conclusion that my argument stands intact and that Professor Hempel's criticisms reveal once more the (...) hopelessness of the position he defends—or rather, of the position he now defends. (shrink)

Aestheticians generally agree that the aesthetic features of an object depend upon the non-aesthetic features of an object, and that this dependence can be captured by some formulation of the supervenience relation. I argue that the aesthetic depends upon the non-aesthetic in various and importantly different ways; that these dependence relations cannot be explained by supervenience; that appeals to supervenience create puzzles that aestheticians have neither fully appreciated nor resolved; and that appealing to various realization relations avoids these puzzles and (...) allows for a richer description of how the aesthetic depends upon the non-aesthetic. (shrink)

This chapter wagers that dance holds a singular, irreducible place in Nancy's work, that it cannot be reduced to thought about dance, and that it provides a way to understanding Nancy's approach to visual culture in general, to equality, and to the circulation of sense in terms of what he calls singular plural being. The chapter takes its starting point from Nancy's discussions of dance in the as yet untranslated Allitérations, a series of email exchanges from 2003 and 2004 followed (...) by transcriptions of face-to-face exchanges between Nancy and choreographer Mathilde Monnier loosely addressing the question of the relation between philosophy and dance, and a conversation between Nancy, Monnier and film-maker Claire Denis. In the first half of the book Nancy raises two questions, each presenting a pair of seemingly irreconcilable demands: 1. How can we understand dance as something open to everyone while retaining the privilege of professional expertise? 2. How can we understand dance in a way that maintains its uniqueness, not reducing it to an example of thought or a vehicle for thought, and yet still allowing for a relation between dance and thought? Both questions invite us to think the privilege of dance together with its openness; its singularity together with its plurality. As Nancy and Monnier grapple with these seemingly contradictory demands in Allitérations, their conversation opens up onto a fresh way to think political equality, and a different way to understand – or ‘catch’, a much more suitable term that I shall introduce later in this chapter – the sense of the world. It is by taking a detour via ‘The Image: Mimesis and Methexis’ that we can also see how Nancy's approach to dance can open to a new way of engaging with visual culture in general. -/- The point in this chapter is not simply to use dance as an example or illustration that helps us better to understand Nancy's approach to visual culture or the Nancean singular plural or his notion of ‘sense’, for in considering dance in terms of art, singular plurality and sense, we shall also be drawn to reassess sense, art and the singular plural in terms of dance. (shrink)

What does it mean to say that music is deeply moving? Or that music's aesthetic value derives from its deep structure? This study traces the widely employed trope of musical depth to its origins in German-language music criticism and analysis. From the Romantic aesthetics of E. T. A. Hoffmann to the modernist theories of Arnold Schoenberg, metaphors of depth attest to the cross-pollination of music with discourses ranging from theology, geology and poetics to psychology, philosophy and economics. The book demonstrates (...) that the persistence of depth metaphors in musicology and music theory today is an outgrowth of their essential role in articulating and transmitting Germanic cultural values. While musical depth metaphors have historically served to communicate German nationalist sentiments, Watkins shows that an appreciation for the broad connotations of those metaphors opens up exciting new avenues for interpretation. (shrink)

Philosophers of science have paid close attention to climate simulations as means of projecting the severity and effects of climate change, but have neglected the full diversity of methods in climate science. This paper shows the philosophical richness of another method in climate science: the practice of using paleoclimate analogues to inform our climate projections. First, I argue that the use of paleoclimate analogues can offer important insights to philosophers of the historical sciences. Rather than using the present as a (...) guide to the past, as is common in the historical sciences, paleoclimate analogues involve using the past as a guide to the future. I thereby distinguish different methods in the historical sciences and argue that these distinctions bear on debates over whether the historical sciences can produce generalizations or predictions. Second, I suggest that paleoclimate analogues might actually be considered a type of climate model, and, as such, their use expands on common characterizations of models to include those that are full-scale, naturally occurring, and non-manipulable. (shrink)

RésuméPaul Boghossian et David Velleman ont soutenu que les théories physicalistes des couleurs — celles qui identifient les propriétés de couleur avec certaines propriétés physiques des objets ou de la lumière—ne peuvent accommoder l'intuition profonde selon laquelle nous ne pouvons pas être dans l'erreur au sujet des contenus représentationnels de nos expériences de couleur. Contre Boghossian et Velleman, je soutiens que cette intuition prétendue que les théories physicalistes ne réussisssent pas à accommoder, nest pas elle-même intuitivement plausible. En fait, c'est (...) clairement un avantage des théories physicalistes que selon elles, les experiences de couleur n'affichent pas avec evidence leurs contenus représentationnels. (shrink)

This is a book about Kant's views on causality as understood in their proper historical context. Specifically, Eric Watkins argues that a grasp of Leibnizian and anti-Leibnizian thought in eighteenth-century Germany helps one to see how the critical Kant argued for causal principles that have both metaphysical and epistemological elements. On this reading Kant's model of causality does not consist of events, but rather of substances endowed with causal powers that are exercised according to their natures and circumstances. This (...) innovative conception of Kant's view of causality casts a light on Kant's philosophical beliefs in general, such as his account of temporality, his explanation of the reconciliation of freedom and determinism, and his response to the skeptical arguments of Hume. (shrink)

Understanding students’ learned hopelessness and academic self-esteem is important because the sense of controllability and competence perception can predict deficits in achievement-oriented behaviours and achievement performance. A survey was conducted to examine the role of learned hopelessness and academic self-esteem in academic achievement. Structural equation modelling was used to analyse the mediational roles of learned hopelessness and academic self-esteem in the academic achievement of 165 Hong Kong junior secondary students. The findings implied that learned hopelessness and academic self-esteem are distinct (...) constructs which play separate mediational roles between prior academic failure and academic achievement. However, the model comparisons indicated that there was no direct effect of learned hopelessness on academic self-esteem or vice versa. (shrink)

This research examined choice behaviour and probability judgement in a counterintuitive reasoning problem called the Monty Hall problem (MHP). In Experiments 1 and 2 we examined whether learning from a simulated card game similar to the MHP affected how people solved the MHP. Results indicated that the experience with the card game affected participants' choice behaviour, in that participants selected to switch in the MHP. However, it did not affect their understanding of the objective probabilities. This suggests that there is (...) dissociation between implicit knowledge gained from the task and the explicit understanding as to why switching was the best strategy. In Experiment 3, the number of prizes and doors were manipulated to examine how participants construed the problem space of the MHP. Results revealed that participants partition the probability judgement to reflect the number of prizes over the number of unopened doors. (shrink)

kant’s critique of pure reason undertakes a systematic investigation of the possibility of synthetic cognition a priori so as to determine whether this kind of cognition is possible in the case of traditional metaphysics.1 While much scholarly attention has been devoted to the distinction between analytic and synthetic judgments as well as to that between the a priori and the a posteriori, less attention has been devoted to understanding exactly what cognition is for Kant. In particular, it is often insufficiently (...) clear what kind of mental state cognition is, what the exact nature of the conditions on cognition is, and how they are satisfied in the case of human beings. To bring greater clarity to... (shrink)

This article argues that Hume's brief essay, "Of the Delicacy of Taste and Passion," offers resources for three claims: (1) Delicate taste correlates with self-sufficiency and thus with a particularly Humean form of Magnanimity -- greatness of mind; (2) Delicate taste improves the capacity for profound friendships, characterized by mutual admiration and true compassion; and (3) magnanimity and compassion are thus not necessarily in tension with one another and may even proceed from and support harmony of character. These claims, in (...) turn, provide reasons to take seriously Hume's view that moral character depends, in part, on aesthetic character. (shrink)

One of the most important thinkers of our time, Jacques Derrida continues to have a profound influence on postmodern thought and society. Christopher Watkin explains Derrida's complex philosophy with clarity and precision, showing not only what Derrida says about metaphysics, ethics, politics, and theology but also what assumptions and commitments underlie his positions. He then brings Derrida into conversation with Reformed theology through the lens of John 1:118, examining both similarities and differences between Derrida and the Bible. Learn why Derrida (...) says what he says and how Christians can receive and respond to his writing in a balanced, biblical way that is truly beneficial to cultural engagement. (shrink)

Karl Raimund Popper was born in Vienna on 18 July 1902. He enrolled at the University of Vienna in 1918 and at the Pedagogic Institute in Vienna in 1925. He was a secondary schoolteacher for several years from 1930 on. His Logik der Forschung was published in 1934. It was brought to Einstein's attention by Frieda Busch, wife of the founder of the Busch Quartet, at the suggestion of her son‐in‐law, the pianist Rudolf Serkin, who was a good friend of (...) Popper's. Einstein told Popper that, purified of certain errors he had noted, the book “will be really splendid”; a considerable correspondence ensued between them. The book also brought Popper invitations to England, where he spent much of 1935–6, meeting Susan Stebbing, Ayer, Berlin, Russell, Ryle, Schrödinger, Woodger, and, at the London School of Economics, Hayek and Robbins. In 1937 he took up a lectureship in philosophy at Canterbury University College, in Christchurch, New Zealand. There he wrote “The poverty of historicism” (1944–5) and The Open Society and its Enemies (1945); he called the latter his “war work.” Both these works contain interesting ideas about the special problems and methods of the social sciences. Later works, including an autobiography (1976), are listed at the end of the chapter. (shrink)

Hugely influential, Michel Foucault's work has not only impacted a diverse range of disciplines—from history and sociology to fine arts, feminism, and gay and lesbian studies—but has also profoundly shaped Western culture at a street level. -/- Yet until now there has been no overarching systematic approach to his work from a Reformed perspective—let alone one that is as fair and accessible as Watkin's. After walking us through key elements of Foucault's thought, Watkin both critiques and answers Foucault through the (...) Bible's teaching on history, power, and identity. His insights are necessary reading for anyone who wants to engage thoughtfully with the ideas of our culture. (shrink)